Structural Analysis of Hypothetical Proteins of Salmonella Typhi CT18

Authors

  • Anila Farid Abbottabad International Medical College, Abbottabad.
  • Saadia Sadiq Abbottabad International Medical College, Abbottabad.
  • Beenish Haider Abbottabad International Medical College, Abbottabad.
  • Adnan Arshad Hazara University, Mansehra.

DOI:

https://doi.org/10.52206/jsmc.2022.12.1.704

Abstract

Background: Salmonella typhi is a gram negative, flagellated, rod shaped facultative, anaerobic bacterium belonging to family
enterobacteriace. It is etiological agent of typhoid fever. It spreads through contamination of water and under cooked food. It has
caused death in many countries where sanitation is poor. It has evolved the ability to spread in deeper tissues of human body..
Objective: To identify the function and structure of hypothetical proteins of Salmonella typhi CT18 by using bioinformatic
programs.
Material and Methods: The 3D structure of these proteins was carried out by the help of Bioinformatics programs such as CMR,
Interproscan, BLAST, Modellor 9.10, Procheck and Prosa. Functional annotation was carried out by using Interproscan and
structure analysis was carried by homology modeling using Modeller.
Results: It was found that there are 4934 proteins are present in Salmonella typhi out of which, 12.1 % were hypothetical proteins.
Among hypothetical proteins, results of 463 proteins were shown by interproscan,6.3% were functionally detected proteins, 2.6%
were uncharacterized proteins and for rest no hits were found. For homology modeling, 5 proteins were selected. The three
dimensional structure of proteins was constructed by Modeller 9.10.
Conclusion: Structural bioinformatics is an effective method to find structure of hypothetical protein which provide good solution
for drug discovery.
Keywords: Homology Modelling, Hypothetical proteins, Typhoid fever,Modellor, Structural Bioinformatics.

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How to Cite

1.
Anila Farid, Saadia Sadiq, Beenish Haider, Adnan Arshad. Structural Analysis of Hypothetical Proteins of Salmonella Typhi CT18. J Saidu Med Coll Swat [Internet]. 2022 Mar. 15 [cited 2025 Jan. 17];12(1):41-6. Available from: http://jsmc.pk/index.php/jsmc/article/view/704

Issue

Vol. 12 No. 1 (2022): Journal of Saidu Medical College, Swat

Section

Original Article

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