Role of Turmeric (Curcuma Longa) in Protection Against Methotrexate Induced Sinusoidal Congestion in the Liver of Albino Mice
DOI:
https://doi.org/10.52206/jsmc.2025.15.1.958Keywords:
Turmeric, Methotrexate, Liver damage, Sinusoidal congestion, AntioxidantAbstract
Background: Methotrexate medication results in hepatotoxicity by free radical accumulation. Curcuma longa commonly known as Turmeric have the antimicrobial, anti-inflammatory, antioxidant, and anti-cancer properties.
Objective: To observe the defensive effects of Turmeric on Methotrexate induced hepatic toxicity in mice.
Materials and Methods: Twenty-eight adult male albino mice were used in this experiment; they were split into four groups: experimental Groups B, C, and D, and control Group A. Four mice were included in Group A, the control group, and eight mice in Group B, C, and D. Group A did not receive any medication Group B received 400 mg/kg of oral Turmeric extract per day for 14 days. On day seven, Group C received an intraperitoneal injection of Methotrexate (40 mg/kg). Group D received 400 mg/kg of Turmeric extract orally every day for 7 days prior to the injection of Methotrexate. On day 7, the injection of 40 mg/kg of Methotrexate was administered intraperitoneally. After that, Group D received 400 mg/kg of Turmeric extract orally every day for an additional 7 days, following the administration of injection Methotrexate. On day 14, every animal was dissected, and the liver was examined under a light microscope to study histological parameters.
Results: The sinusoids appeared normal in the liver sections of group A (control) according to the histological analysis. Group B, which received treatment with Turmeric, did not exhibit any discernible histological alterations. Upon examining the liver tissue of Group C (the group receiving Methotrexate), severe histological alterations were observed, which included sinusoidal congestion. Group D (Methotrexate + Turmeric group) demonstrated a significant reduction in sinusoidal congestion.
Conclusion: This study concluded Turmeric (Curcuma longa) protected liver tissue against methotrexate. induced damage.
Keywords: Antioxidant, Liver damage, Methotrexate, Sinusoidal congestion, Turmeric.
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