Effect of Administration of Neurokinin B Agonist (Senktide) and Antagonist (Sb222200) on Hematological and Coagulation Parameters in Adult Male Rats
DOI:
https://doi.org/10.52206/jsmc.2025.15.1.986Abstract
Background: Neurokinin B is known as a regulator of reproduction, however its chronic effects on hematological and coagulation parameters in adult male mammals are not well understood
Objective: To explore the effects of Senktide (Neurokinin B agonist) and SB222200 (Neurokinin B antagonist) administration on hematological and coagulation parameters in adult rats.
Materials and Methods: This laboratory-based experimental study was conducted at Gomal University and KMU Institute of Medical Sciences from February 2019 to April 2021. Healthy adult male Sprague Dawley rats were randomly assigned to three groups (n=5 animals/group). They were administered intraperitoneally with Senktide (1µg) and SB222200 (1µg) consecutively twice daily for 12 days. Control rats received distilled water injections. The animals were sacrificed after 12-day treatment. Blood samples were collected directly from the left ventricle to analyze hematological and coagulation parameters.
Results: Senktide-treated rats showed significantly prolonged bleeding time (111±17.10sec) compared to SB222200 (27±12.55sec) and control (33±12.55sec). Clotting time was also higher (Senktide: 96±17.10sec; SB222200: 39±8.22sec; control: 42±12.55sec), as were prothrombin time [PT] (Senktide: 90±14.58sec; SB222200: 15.80±3.90sec; control: 15±4.12sec), activated partial thromboplastin time [APPT] (Senktide: 92±25.25sec; SB222200: 16.20±3.19sec; control: 19.40±2.97sec) and international normalized ratio (Senktide: 6.60±1.07; SB222200: 1.16±0.29; control: 1.10±0.30) in Senktide-treated rats. Mean platelet volume (Senktide: 8.88±0.33fl; SB222200: 7.92±0.29fl; control: 8.04±0.48fl) and leukocyte count (×10³/µl) (Senktide: 12±0.79; SB222200: 8.89±0.97; control: 8.87±1.32) were significantly elevated however platelet count (×10³/µl) (Senktide: 792.20±50.39; SB222200: 968.60±45.33; control: 960.60±58.71) was reduced in Senktide-treated rats.
Conclusion: Senktide administration led to leukocytosis, thromboctopenia, and affected coagulation pathways with prolonged BT, CT, PT and APTT.
Keywords: Activated Partial Thromboplastin Time, Blood Coagulation, Blood Coagulation Tests, Neurokinin B, Platelets, Prothrombin Time, Senktide, SB222200, Tachykinins.
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